About Fish Embryo Acute Toxicity Test
This test is designed to determine acute toxicity of chemicals on embryonic stages of zebrafish (Danio rerio). The embryonic stages of fish are considered not to perceive pain compared to adult fish. Therefore, the Fish Embroy Acute Toxicity Test is used as an alternative for an animal test.
The test uses newly fertilized zebrafish eggs to be exposed to the test chemicals for 96 hours and observe it every 24 hours. The lethality of the test chemicals are recorded by the four observations which are as followed:
- Coagulation of fertilised eggs
- Lack of somite formation
- Lack of detachment of the tail-bud from the yolk sac, and
- Lack of heartbeat.
After the 96 hours of exposure is ended, the outcomes from the four observations will determine the acute toxicity of the test chemicals. Afterwards, the lethal dose (LC50) is calculated.