About Skin Corrosion Test
Skin corrosion is irreversible damage to skin after skin is exposed to or in contact with corrosive chemical substances or mixers. It appears as visible necrosis which is the death of almost or all cells in tissue because of disease or injury. Skin corrosion is more severe than skin irritation as the damage cannot be reversed because the damage does not only happen at the epidermis which is the outermost layer of skin, it also goes through into the dermis which is beneath the epidermis.
The common symptoms of skin corrosion are associated with blackened or dead skin, swelling, numbness, blisters, pain, fluid collection, skin discoloration, redness of skin or burning in the affected area.
Skin Corrosion Test is an in vitro test designed to discriminate corrosive and non-corrosive chemical substances or ingredients that lead to skin corrosion in accordance with the UN Globally Harmonized System of Classification and Labelling (GHS) Category 1. It is a non-animal test which is based on the in vitro test system of reconstructed human epidermis (RHE), which closely imitates the biochemical and physiological properties of the upper parts of the human skin. The test system uses non-transformed keratinocytes, which is derived from humans, as a cell source to build an epidermal model with representatives of histology and cytoarchitecture.
6 Validated RhE test methods for Skin Corrosion Test
There are six validated RhE test methods for skin corrosion test approved by OECD. They are as followed:
- EpiSkin™ (SM)
- EpiDerm™ SCT (EPI-200)
- SkinEthic™ RHE
- LabCyte EPI-MODEL24 SCT