About Skin Corrosion Test
Skin corrosion refers to the irreversible damage caused to the skin when exposed to or in contact with corrosive chemical substances or mixtures. This damage manifests as visible necrosis, which involves the death of most or all cells in the affected tissue due to injury or disease. Skin corrosion is more severe than skin irritation because it cannot be reversed, as it affects not only the outermost layer of skin, known as the epidermis, but also extends into the deeper layer called the dermis.
Typical symptoms of skin corrosion include blackened or dead skin, swelling, numbness, blisters, pain, fluid accumulation, skin discoloration, redness, or a burning sensation in the affected area.
The Skin Corrosion Test is an in vitro test specifically designed to differentiate between corrosive and non-corrosive chemical substances or ingredients, in accordance with the UN Globally Harmonized System of Classification and Labelling (GHS) Category 1. This test is a non-animal alternative that utilizes a reconstructed human epidermis (RHE) model, closely resembling the biochemical and physiological properties of the upper layers of human skin. The test employs non-transformed keratinocytes derived from humans as the cell source to construct an epidermal model with representative histology and cytoarchitecture.
6 Validated RhE test methods for Skin Corrosion Test
There are six validated RhE test methods for the Skin Corrosion Test, as approved by the OECD. These methods are as follows:
• EpiSkin™ (SM)
• EpiDerm™ SCT (EPI-200)
• SkinEthic™ RHE
• LabCyte EPI-MODEL24 SCT