What is a Skin Irritation Test?
Skin Irritation Test is an in vitro test used for identifying hazard chemical substances or ingredients that cause skin irritation in accordance with the UN Globally Harmonized System of Classification and Labelling (GHS) Category 2. It is a non-animal test which is based on the in vitro test system of reconstructed human epidermis (RHE), which closely imitates the biochemical and physiological properties of the upper parts of the human skin.
The test system uses non-transformed keratinocytes, which is derived from humans, as a cell source to build an epidermal model with representatives of histology and cytoarchitecture. Whether a chemical substance or ingredient has a skin irritation potential or not is determined based on tissue viability. When an epidermal model is exposed to a test chemical substance and the viability of the tested chemical substance is reduced to 50% or below 50% of the mean viability of negative controls, then the tested chemical substance is classified as an irritant in accordance with UN GHS Category 2.
We provide 6 validated RhE test methods for Skin Irritation Test
There are six validated RhE test methods for the skin irritation test approved by OECD. They are as followed:
- EpiSkin™ (SM)
- EpiDerm™ SIT (EPI-200)
- SkinEthic™ RHE
- LabCyte EPI-MODEL24 SIT